Temporal σ^B stress-response profiles impact Bacillus subtilis fitness.

ABSTRACT: The Gram-positive model organism Bacillus subtilis responds to environmental stressors by activating the alternative sigma factor σ^B. The sensing apparatus upstream of σ^B activation is thought to consist of cytoplasmic stressosomes-megadalton-sized protein complexes that include five paralogous proteins known as RsbRs. The RsbRs are presumed to be involved in stress sensing and the subsequent response. Perturbations to the RsbR complement in stressosomes by engineering cells that produce only one of the RsbR paralogs ("single-RsbR strains") lead to altered σ^B response dynamics with respect to timing and magnitude. Here, we asked whether such changes to σ^B response dynamics impact the relative fitness of a strain. We competed strain pairs w

SUBMITTER: Bush SR 

PROVIDER: S-EPMC10900884 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature