Rational Formulation of targeted ABT-737 nanoparticles by self-assembled polypeptides and designed peptides.

ABSTRACT: Here we present the development of nanoparticles (NPs) formulations specifically designed for targeting the antiapoptotic Bcl-2 proteins on the outer membrane of mitochondria with the drug agent ABT-737. The NPs which are self-assembled by the natural polypeptide poly gamma glutamic acid (ϒPGA) and a designed cationic and amphiphilic peptide (PFK) have been shown to target drugs toward mitochondria. In this study we systematically developed the formulation of such NPs loaded with the ABT-737 and demonstrated the cytotoxic effect of the best identified formulation on MDA-MB-231 cells. Our findings emphasize the critical role of solutions pH and the charged state of the components throughout the formulation process as well as the concentrations of the co-components and their mixing sequence,

SUBMITTER: Aibinder P 

PROVIDER: S-EPMC10900936 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature