Integrative temporal multi-omics reveals uncoupling of transcriptome and proteome during human T cell activation.

ABSTRACT: Engagement of the T cell receptor (TCR) triggers molecular reprogramming leading to the acquisition of specialized effector functions by CD4 helper and CD8 cytotoxic T cells. While transcription factors, chemokines, and cytokines are known drivers in this process, the temporal proteomic and transcriptomic changes that regulate different stages of human primary T cell activation remain to be elucidated. Here, we report an integrative temporal proteomic and transcriptomic analysis of primary human CD4 and CD8 T cells following ex vivo stimulation with anti-CD3/CD28 beads, which revealed major transcriptome-proteome uncoupling. The early activation phase in both CD4 and CD8 T cells was associated with transient downregulation of the mRNA transcripts and protein of the central glucose transpor

SUBMITTER: Weerakoon H 

PROVIDER: S-EPMC10901835 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature