Small extracellular vesicles in follicular fluids for predicting reproductive outcomes in assisted reproductive technology.

ABSTRACT:

Background

Assisted reproductive technology accounts for an increasing proportion of infertility treatments, and assessments to predict clinical pregnancy outcomes are desired. Extracellular vesicles exist in follicular fluid, and small non coding RNAs in extracellular vesicles underline the possibility of reflecting pregnancy potential.

Methods

Follicular fluid samples are collected from 20 ovarian follicles of 15 infertile patients undergoing assisted reproductive technology. Extracellular vesicles are isolated by serial centrifugation and small RNA sequencing is performed to investigate the profiles of microRNAs and P-element-induced wimpy testis-interacting RNAs.

Results

Small extracellular vesicles with a size range of approximately 100 nm are successfully isolat

SUBMITTER: Muraoka A 

PROVIDER: S-EPMC10902298 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature