The effect of phytosulfokine alpha on haploid embryogenesis and gene expression of Brassica napus microspore cultures.

ABSTRACT: Microspore embryogenesis (ME) is the most powerful tool for creating homozygous lines in plant breeding and molecular biology research. It is still based mainly on the reprogramming of microspores by temperature, osmotic and/or nutrient stress. New compounds are being sought that could increase the efficiency of microspore embryogenesis or even induce the formation of haploid embryos from recalcitrant genotypes. Among these, the mitogenic factor phytosulfokine alpha (PSK-α) is promising due to its broad spectrum of activity in vivo and in vitro. The aim of our study was to investigate the effect of PSK-α on haploid embryogenesis from microspores of oilseed rape (Brassica napus L., DH4079), one of the most important oil crops and a model plant for studying the molecular

SUBMITTER: Mestinsek Mubi S 

PROVIDER: S-EPMC10902448 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature