Dataset Information

Taenia martis Neurocysticercosis-Like Lesion in Child, Associated with Local Source, the Netherlands.

ABSTRACT: A neurocysticercosis-like lesion in an 11-year-old boy in the Netherlands was determined to be caused by the zoonotic Taenia martis tapeworm. Subsequent testing revealed that 15% of wild martens tested in that region were infected with T. martis tapeworms with 100% genetic similarity; thus, the infection source was most likely local.

SUBMITTER: Eggink H

PROVIDER: S-EPMC10902551 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature

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Taenia martis Neurocysticercosis-Like Lesion in Child, Associated with Local Source, the Netherlands.

Eggink Hendriekje H Maas Miriam M van den Brand Judith M A JMA Dekker Jasja J Franssen Frits F Hoving Eelco W EW Kortbeek Laetitia M LM Kranendonk Mariëtte E G MEG Meiners Linda C LC Rittscher Anne E AE Roelfsema Jeroen J Schölvinck Elisabeth H EH

Emerging infectious diseases 20240301 3

A neurocysticercosis-like lesion in an 11-year-old boy in the Netherlands was determined to be caused by the zoonotic Taenia martis tapeworm. Subsequent testing revealed that 15% of wild martens tested in that region were infected with T. martis tapeworms with 100% genetic similarity; thus, the infection source was most likely local. ...[more]

PMID: 38407150

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Project description:BackgroundAntigen tests for diagnosis and disease monitoring in some types of neurocysticercosis (NCC) are useful but access to testing has been limited by availability of proprietary reagents and/or kits.Methods/principal findingsThree previously identified IgM-secreting hybridomas whose IgM products demonstrated specificity to Taenia solium underwent variable heavy and light chain sequencing and isotype conversion to mouse IgG. Screening of these recombinantly expressed IgG anti-Ts hybridomas, identified one (TsG10) with the highest affinity to crude Taenia antigen. TsG10 was then used as a capture antibody in a sandwich antigen detection immunoassay in combination with either a high titer polyclonal anti-Ts antibody or with biotinylated TsG10 (termed TsG10*bt). Using serum, plasma, and CSF samples from patients with active NCC and those from NCC-uninfected patients, ROC curve analyses demonstrated that the TsG10-TsG10-*bt assay achieved a 98% sensitivity and 100% specificity in detecting samples known to be antigen positive and outperformed the polyclonal based assay (sensitivity of 93% with 100% specificity). By comparing levels of Ts antigen (Ag) in paired CSF (n = 10) or plasma/serum (n = 19) samples from well-characterized patients with extra-parenchymal NCC early in infection and at the time of definitive cure, all but 2 (1 from CSF and 1 from plasma) became undetectable. There was a high degree of correlation (r = 0.98) between the Ag levels detected by this new assay and levels found by a commercial assay. Pilot studies indicate that this antigen can be detected in the urine of patients with active NCC.Conclusions/significanceA newly developed recombinant monoclonal antibody-based Ts Ag detection immunoassay is extremely sensitive in the detection of extra-parenchymal NCC and can be used to monitor the success of treatment in the CSF, serum/plasma and urine. The ability to produce recombinant TsG10 at scale should enable use of this antigen detection immunoassay wherever NCC is endemic.Clinical trial registrationClinicalTrials.gov Identifiers: NCT00001205 - & NCT00001645.

Dataset Information

Taenia martis Neurocysticercosis-Like Lesion in Child, Associated with Local Source, the Netherlands.

Publications

Taenia martis Neurocysticercosis-Like Lesion in Child, Associated with Local Source, the Netherlands.

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