Multiscale transport and 4D time-lapse imaging in precision-cut liver slices (PCLS).

ABSTRACT:

Background

Monitoring cellular processes across different levels of complexity, from the cellular to the tissue scale, is important for understanding tissue structure and function. However, it is challenging to monitor and estimate these structural and dynamic interactions within three-dimensional (3D) tissue models.

Objective

The aim of this study was to design a method for imaging, tracking, and quantifying 3D changes in cell morphology (shape and size) within liver tissue, specifically a precision-cut liver slice (PCLS). A PCLS is a 3D model of the liver that allows the study of the structure and function of liver cells in their native microenvironment.

Methods

Here, we present a method for imaging liver tissue during anisosmotic exposure in a multispectral four-di

SUBMITTER: Azam I 

PROVIDER: S-EPMC10903333 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature