Evaluating the sensitivity of droplet digital PCR for the quantification of SARS-CoV-2 in wastewater.

ABSTRACT: Wastewater surveillance for SARS-CoV-2 has been demonstrated to be a valuable tool in monitoring community-level virus circulation and assessing new outbreaks. It may become a useful tool in the early detection and response to future pandemics, enabling public health authorities to implement timely interventions and mitigate the spread of infectious diseases with the fecal excretion of their agents. It also offers a chance for cost-effective surveillance. Reverse transcription-quantitative polymerase chain reaction (RTqPCR) is the most commonly used method for viral RNA detection in wastewater due to its sensitivity, reliability, and widespread availability. However, recent studies have indicated that reverse transcription droplet digital PCR (RTddPCR) has the potential to offer improved s

SUBMITTER: de la Cruz Barron M 

PROVIDER: S-EPMC10903512 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature