Quantification of fibrosis extend and airspace availability in lung: A semi-automatic ImageJ/Fiji toolbox.

ABSTRACT: The evaluation of the structural integrity of mechanically dynamic organs such as lungs is critical for the diagnosis of numerous pathologies and the development of therapies. This task is classically performed by histology experts in a qualitative or semi-quantitative manner. Automatic digital image processing methods appeared in the last decades, and although immensely powerful, tools are highly specialized and lack the versatility required in various experimental designs. Here, a set of scripts for the image processing software ImageJ/Fiji to easily quantify fibrosis extend and alveolar airspace availability in Sirius Red or Masson's trichrome stained samples is presented. The toolbox consists in thirteen modules: sample detection, particles filtration (automatic and manual), border def

SUBMITTER: Segard BD 

PROVIDER: S-EPMC10903859 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature