Single-cell analysis of isoform switching and transposable element expression during preimplantation embryonic development.

ABSTRACT: Alternative splicing is an essential regulatory mechanism for development and pathogenesis. Through alternative splicing one gene can encode multiple isoforms and be translated into proteins with different functions. Therefore, this diversity is an important dimension to understand the molecular mechanism governing embryo development. Isoform expression in preimplantation embryos has been extensively investigated, leading to the discovery of new isoforms. However, the dynamics of isoform switching of different types of transcripts throughout the development remains unexplored. Here, using single-cell direct isoform sequencing in over 100 single blastomeres from the mouse oocyte to blastocyst stage, we quantified isoform expression and found that 3-prime partial transcripts lacking stop cod

SUBMITTER: Wang C 

PROVIDER: S-EPMC10903961 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature