Engraftment of adult hematopoietic stem and progenitor cells in a novel model of humanized mice.

ABSTRACT: Pre-clinical use of humanized mice transplanted with CD34⁺ hematopoietic stem and progenitor cells (HSPCs) is limited by insufficient engraftment with adult non-mobilized HSPCs. Here, we developed a novel immunodeficient mice based on NOD-SCID-Il2γc^-/- (NSG) mice to support long-term engraftment with human adult HSPCs. As both Flt3L and IL-6 are critical for many aspects of hematopoiesis, we knock-out mouse Flt3 and knock-in human IL6 gene. The resulting mice showed an increase in the availability of mouse Flt3L to human cells and a dose-dependent production of human IL-6 upon activation. Upon transplantation with low number of human HSPCs from adult bone marrow, these humanized mice demonstrated a significantly higher engraftment with multilineag

SUBMITTER: Yu CI 

PROVIDER: S-EPMC10904995 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature