Activation of ductal progenitor-like cells from adult human pancreas requires extracellular matrix protein signaling.

ABSTRACT: Ductal progenitor-like cells are a sub-population of ductal cells in the adult human pancreas that have the potential to contribute to regenerative medicine. However, the microenvironmental cues that regulate their activation are poorly understood. Here, we establish a 3-dimensional suspension culture system containing six defined soluble factors in which primary human ductal progenitor-like and ductal non-progenitor cells survive but do not proliferate. Expansion and polarization occur when suspension cells are provided with a low concentration (5% v/v) of Matrigel, a sarcoma cell product enriched in many extracellular matrix (ECM) proteins. Screening of ECM proteins identified that collagen IV can partially recapitulate the effects of Matrigel. Inhibition of integrin α1β1, a major collag

SUBMITTER: Zook HN 

PROVIDER: S-EPMC10904999 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature