Hypoxia macrophage-derived exosomal miR-26b-5p targeting PTEN promotes the development of keloids.

ABSTRACT:

Background

Hypoxia is the typical characteristic of keloids. The development of keloids is closely related to the abnormal phenotypic transition of macrophages. However, the role of exosomal microRNAs (miRNAs) derived from hypoxic macrophages in keloids remains unclear. This study aimed to explore the role of hypoxic macrophage-derived exosomes (HMDE) in the occurrence and development of keloids and identify the critical miRNA.

Methods

The expression of CD206⁺ M2 macrophage in keloids and normal skin tissues was examined through immunofluorescence. The polarization of macrophages under a hypoxia environment was detected through flow cytometry. The internalization of macrophage-derived exosomes in human keloid fibroblasts (HKFs) was detected using a confocal micros

SUBMITTER: Dai S 

PROVIDER: S-EPMC10905499 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature