A drug-selectable acoustic reporter gene system for human cell ultrasound imaging.

ABSTRACT: A promising new field of genetically encoded ultrasound contrast agents in the form of gas vesicles has recently emerged, which could extend the specificity of medical ultrasound imaging. However, given the delicate genetic nature of how these genes are integrated and expressed, current methods of producing gas vesicle-expressing mammalian cell lines requires significant cell processing time to establish a clonal/polyclonal line that robustly expresses the gas vesicles sufficiently enough for ultrasound contrast. Here, we describe an inducible and drug-selectable acoustic reporter gene system that can enable gas vesicle expression in mammalian cell lines, which we demonstrate using HEK293T cells. Our drug-selectable construct design increases the stability and proportion of cells that succ

SUBMITTER: Howells AR 

PROVIDER: S-EPMC10905554 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature