Goat uromodulin promoter directs kidney-specific expression of GFP gene in transgenic mice.

ABSTRACT:

Background

Uromodulin is the most abundant protein found in the urine of mammals. In an effort to utilize the uromodulin promoter in order to target recombinant proteins in the urine of transgenic animals we have cloned a goat uromodulin gene promoter fragment (GUM promoter) and used it to drive expression of GFP in the kidney of transgenic mice.

Results

The GUM-GFP cassette was constructed and transgenic mice were generated in order to study the promoter's tissue specificity, the GFP kidney specific expression and its subcellular distribution. Tissues collected from three GUM-GFP transgenic mouse lines, and analyzed for the presence of GFP by Western blotting and fluorescence confirmed that the GUM promoter drove expression of GFP specifically in the kidney. More specifical

SUBMITTER: Huang YJ 

PROVIDER: S-EPMC1090560 | biostudies-literature | 2005 Apr

REPOSITORIES: biostudies-literature