Expanding the evo-devo toolkit: generation of 3D mammary tissue from diverse mammals.

ABSTRACT: The varying pathways of mammary gland development across species and evolutionary history are underexplored, largely due to a lack of model systems. Recent progress in organoid technology holds the promise of enabling in-depth studies of the developmental adaptations that have occurred throughout the evolution of different species, fostering beneficial phenotypes. The practical application of this technology for mammary glands has been mostly confined to rodents and humans. In the current study, we have successfully created next-generation 3D mammary gland organoids from eight eutherian mammals and the first branched organoid of a marsupial mammary gland. Using mammary organoids, we identified a role for ROCK protein in regulating branching morphogenesis, a role that manifests differently

SUBMITTER: Kim HY 

PROVIDER: S-EPMC10905751 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature