Nanopore targeted sequencing-based diagnosis of central nervous system infections in HIV-infected patients.

ABSTRACT:

Background

Early and accurate etiological diagnosis is very important for improving the prognosis of central nervous system (CNS) infections in human immunodeficiency virus (HIV)-infected patients. The goal is not easily achieved by conventional microbiological tests. We developed a nanopore targeted sequencing (NTS) platform and evaluated the diagnostic performance for CNS infections in HIV-infected patients, with special focus on cryptococcal meningitis (CM). We compared the CM diagnostic performance of NTS with conventional methods and cryptococcal polymerase chain reaction (PCR).

Methods

This study included 57 hospitalized HIV-infected patients with suspected CNS infections from September 2018 to March 2022. The diagnosis established during hospitalization includes 27 ca

SUBMITTER: Yang X 

PROVIDER: S-EPMC10905896 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature