Artificial Targets: a versatile cell-free platform to characterize CAR T cell function in vitro.

ABSTRACT: Cancer immunotherapies using chimeric antigen receptor (CAR) T cells have tremendous potential and proven clinical efficacy against a number of malignancies. Research and development are emerging to deepen the knowledge of CAR T cell efficacy and extend the therapeutic potential of this novel therapy. To this end, functional characterization of CAR T cells plays a central role in consecutive phases across fundamental research and therapeutic development, with increasing needs for standardization. The functional characterization of CAR T cells is typically achieved by assessing critical effector functions, following co-culture with cell lines expressing the target antigen. However, the use of target cell lines poses several limitations, including alterations in cell fitness, metabolic state

SUBMITTER: Wang X 

PROVIDER: S-EPMC10906080 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature