Intracellular localization of Crimean-Congo Hemorrhagic Fever (CCHF) virus glycoproteins.

ABSTRACT:

Background

Crimean-Congo Hemorrhagic Fever virus (CCHFV), a member of the genus Nairovirus, family Bunyaviridae, is a tick-borne pathogen causing severe disease in humans. To better understand the CCHFV life cycle and explore potential intervention strategies, we studied the biosynthesis and intracellular targeting of the glycoproteins, which are encoded by the M genome segment.

Results

Following determination of the complete genome sequence of the CCHFV reference strain IbAr10200, we generated expression plasmids for the individual expression of the glycoproteins GN and GC, using CMV- and chicken beta-actin-driven promoters. The cellular localization of recombinantly expressed CCHFV glycoproteins was compared to authentic glycoproteins expressed during virus infection using

SUBMITTER: Haferkamp S 

PROVIDER: S-EPMC1090624 | biostudies-literature | 2005 Apr

REPOSITORIES: biostudies-literature