Identification and characterization of the mediator kinase-dependent myometrial stem cell phosphoproteome.

ABSTRACT:

Objective

To identify, in myometrial stem/progenitor cells, the presumptive cell of origin for uterine fibroids, substrates of Mediator-associated cyclin dependent kinase 8/19 (CDK8/19), which is known to be disrupted by uterine fibroid driver mutations in Mediator complex subunit 12 (MED12).

Design

Experimental study.

Setting

Academic research laboratory.

Patient(s)

Women undergoing hysterectomy for uterine fibroids.

Intervention(s)

Stable isotopic labeling of amino acids in cell culture (SILAC) coupled with chemical inhibition of CDK8/19 and downstream quantitative phosphoproteomics and transcriptomic analyses in myometrial stem/progenitor cells.

Main outcome measure(s)

High-confidence Mediator kinase substrates identified by SILAC-based quantit

SUBMITTER: Barron L 

PROVIDER: S-EPMC10906282 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature