Protein Translocation Activity in Surface-Supported Lipid Bilayers.

ABSTRACT: Surface-supported lipid bilayers are used widely throughout the nanoscience community as cellular membrane mimics. For example, they are frequently employed in single-molecule atomic force microscopy (AFM) studies to shed light on membrane protein conformational dynamics and folding. However, in AFM as well as in other surface-sensing techniques, the close proximity of the supporting surface raises questions about preservation of the biochemical activity. Employing the model translocase from the general secretory (Sec) system of Escherichia coli, here we quantify the activity via two biochemical assays in surface-supported bilayers. The first assesses ATP hydrolysis and the second assesses polypeptide translocation across the membrane via protection from added protease. Hydrolysis a

SUBMITTER: Chattrakun K 

PROVIDER: S-EPMC10906442 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature