The <i>dabABC</i> operon is a marker of C4-alkylated monobactam biosynthesis and responsible for (<i>2S</i>,<i>3R</i>)-diaminobutyrate production.

ABSTRACT: Non-ribosomal peptide synthetases (NRPSs) assemble metabolites of medicinal and commercial value. Both serine and threonine figure prominently in these processes and separately can be converted to the additional NRPS building blocks 2,3-diaminopropionate (Dap) and 2,3-diaminobutyrate (Dab). Here we bring extensive bioinformatics, in vivo and in vitro experimentation to compose a unified view of the biosynthesis of these widely distributed non-canonical amino acids that both derive by pyridoxal-mediated β-elimination of the activated O-phosphorylated substrates followed by β-addition of an amine donor. By examining monobactam biosynthesis in Pseudomonas and in Burkholderia species where it is silent, we show that (2S,3R)-Dab synthesis depends

SUBMITTER: Li R 

PROVIDER: S-EPMC10906522 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature