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Protocol for synthesis of spherical silver nanoparticles with stable optical properties and characterization by transmission electron microscopy.


ABSTRACT: The synthesis of metallic plasmonic nanoparticles (NPs) faces challenges in stability and reproducibility, especially with silver. Here, we present a protocol for tunable synthesis of spherical silver NPs (AgNPs) with stable optical properties. We describe steps for preparing solutions, morphological characterization of AgNPs by transmission electron microscopy, and testing stability. AgNPs exhibit enduring stability and compatibility with various pH values. Moreover, they can be functionalized for optical biosensing applications, offering versatility in nanomaterial applications.

SUBMITTER: Nocerino V 

PROVIDER: S-EPMC10906526 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature

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Protocol for synthesis of spherical silver nanoparticles with stable optical properties and characterization by transmission electron microscopy.

Nocerino Valeria V   Miranda Bruno B   Dardano Principia P   Sanità Gennaro G   Esposito Emanuela E   De Stefano Luca L  

STAR protocols 20240223 1


The synthesis of metallic plasmonic nanoparticles (NPs) faces challenges in stability and reproducibility, especially with silver. Here, we present a protocol for tunable synthesis of spherical silver NPs (AgNPs) with stable optical properties. We describe steps for preparing solutions, morphological characterization of AgNPs by transmission electron microscopy, and testing stability. AgNPs exhibit enduring stability and compatibility with various pH values. Moreover, they can be functionalized  ...[more]

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