Human surface ectoderm and amniotic ectoderm are sequentially specified according to cellular density.

ABSTRACT: Mechanisms specifying amniotic ectoderm and surface ectoderm are unresolved in humans due to their close similarities in expression patterns and signal requirements. This lack of knowledge hinders the development of protocols to accurately model human embryogenesis. Here, we developed a human pluripotent stem cell model to investigate the divergence between amniotic and surface ectoderms. In the established culture system, cells differentiated into functional amnioblast-like cells. Single-cell RNA sequencing analyses of amnioblast differentiation revealed an intermediate cell state with enhanced surface ectoderm gene expression. Furthermore, when the differentiation started at the confluent condition, cells retained the expression profile of surface ectoderm. Collectively, we propose that

SUBMITTER: Nakanoh S 

PROVIDER: S-EPMC10906920 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature