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Diverse roles of pontine NPS-expressing neurons in sleep regulation.

ABSTRACT: Neuropeptide S (NPS) was postulated to be a wake-promoting neuropeptide with unknown mechanism, and a mutation in its receptor (NPSR1) causes the short sleep duration trait in humans. We investigated the role of different NPS⁺ nuclei in sleep/wake regulation. Loss-of-function and chemogenetic studies revealed that NPS⁺ neurons in the parabrachial nucleus (PB) are wake-promoting, whereas peri-locus coeruleus (peri-LC) NPS⁺ neurons are not important for sleep/wake modulation. Further, we found that a NPS⁺ nucleus in the central gray of the pons (CGPn) strongly promotes sleep. Fiber photometry recordings showed that NPS⁺ neurons are wake-active in the CGPn and wake/REM-sleep active in the PB and peri-LC. Blocking NPS-NPSR1 signaling or knockdown of Nps supported the function of the NPS-NPSR1 pathway in sleep/wake regulation. Together, these results reveal that NPS and NPS⁺ neurons play dichotomous roles in sleep/wake regulation at both the molecular and circuit levels.

SUBMITTER: Xing L

PROVIDER: S-EPMC10907243 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature

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Diverse roles of pontine NPS-expressing neurons in sleep regulation.

Xing Lijuan L Zou Xianlin X Yin Chen C Webb John M JM Shi Guangsen G Ptáček Louis J LJ Fu Ying-Hui YH

Proceedings of the National Academy of Sciences of the United States of America 20240221 9

Neuropeptide S (NPS) was postulated to be a wake-promoting neuropeptide with unknown mechanism, and a mutation in its receptor (NPSR1) causes the short sleep duration trait in humans. We investigated the role of different NPS+ nuclei in sleep/wake regulation. Loss-of-function and chemogenetic studies revealed that NPS+ neurons in the parabrachial nucleus (PB) are wake-promoting, whereas peri-locus coeruleus (peri-LC) NPS+ neurons are not important for sleep/wake ...[more]

PMID: 38381789

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Diverse roles of pontine NPS-expressing neurons in sleep regulation.

Publications

Diverse roles of pontine NPS-expressing neurons in sleep regulation.

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