Characterization of SRp46, a novel human SR splicing factor encoded by a PR264/SC35 retropseudogene.

ABSTRACT: The highly conserved SR family contains a growing number of phosphoproteins acting as both essential and alternative splicing factors. In this study, we have cloned human genomic and cDNA sequences encoding a novel SR protein designated SRp46. Nucleotide sequence analyses have revealed that the SRp46 gene corresponds to an expressed PR264/SC35 retropseudogene. As a result of mutations and amplifications, the SRp46 protein significantly differs from the PR264/SC35 factor, mainly at the level of its RS domain. Northern and Western blot analyses have established that SRp46 sequences are expressed at different levels in several human cell lines and normal tissues, as well as in simian cells. In contrast, sequences homologous to SRp46 are not present in mice. In vitro splicing studies indicate

SUBMITTER: Soret J 

PROVIDER: S-EPMC109076 | biostudies-literature | 1998 Aug

REPOSITORIES: biostudies-literature