O-GlcNAc of STING mediates antiviral innate immunity.

ABSTRACT:

Background

O-GlcNAcylation modification affects multiple physiological and pathophysiolocal functions of cells. Altered O-GlcNAcylation was reported to participate in antivirus response. Stimulator of interferon genes (STING) is an adaptor mediating DNA virus-induced innate immune response. Whether STING is able to be modified by O-GlcNAcylation and how O-GlcNAcylation affects STING-mediated anti-DNA virus response remain unknown.

Methods

Metabolomics analysis was used for detecting metabolic alterations in HSV-1 infection cells. Succinylated wheat germ agglutinin (sWGA), co-immunoprecipitation, and pull-down assay were employed for determining O-GlcNAcylation. Mutagenesis PCR was applied for the generation of STING mutants. WT and Sting1^-/- C57BL/6 mice (KOCMP-72

SUBMITTER: Li Y 

PROVIDER: S-EPMC10908090 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature