Newly imported proteins in mitochondria are particularly sensitive to aggregation.

ABSTRACT:

Aim

A functional proteome is essential for life and maintained by protein quality control (PQC) systems in the cytosol and organelles. Protein aggregation is an indicator of a decline of PQC linked to aging and disease. Mitochondrial PQC is critical to maintain mitochondrial function and thus cellular fitness. How mitochondria handle aggregated proteins is not well understood. Here we tested how the metabolic status impacts on formation and clearance of aggregates within yeast mitochondria and assessed which proteins are particularly sensitive to denaturation.

Methods

Confocal microscopy, electron microscopy, immunoblotting and genetics were applied to assess mitochondrial aggregate handling in response to heat shock and ethanol using the mitochondrial disaggregase Hsp78 as

SUBMITTER: Vazquez-Calvo C 

PROVIDER: S-EPMC10909475 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature