Renal arterial infusion of tempol prevents medullary hypoperfusion, hypoxia, and acute kidney injury in ovine Gram-negative sepsis.

ABSTRACT:

Aim

Renal medullary hypoperfusion and hypoxia precede acute kidney injury (AKI) in ovine sepsis. Oxidative/nitrosative stress, inflammation, and impaired nitric oxide generation may contribute to such pathophysiology. We tested whether the antioxidant and anti-inflammatory drug, tempol, may modify these responses.

Methods

Following unilateral nephrectomy, we inserted renal arterial catheters and laser-Doppler/oxygen-sensing probes in the renal cortex and medulla. Noanesthetized sheep were administered intravenous (IV) Escherichia coli and, at sepsis onset, IV tempol (IVT; 30 mg kg^-1  h^-1 ), renal arterial tempol (RAT; 3 mg kg^-1  h^-1 ), or vehicle.

Results

Septic sheep receiving vehicle developed renal medullary hypoperfusion

SUBMITTER: Betrie AH 

PROVIDER: S-EPMC10909540 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature