Kinetic modelling of the cellular metabolic responses underpinning in vitro glycolysis assays.

ABSTRACT: This study aims to demonstrate the benefits of augmenting commercially available, real-time, in vitro glycolysis assays with phenomenological rate equation-based kinetic models, describing the contributions of the underpinning metabolic pathways. To this end, a commercially available glycolysis assay, sensitive to changes in extracellular acidification (extracellular pH), was used to derive the glycolysis pathway kinetics. The pathway was numerically modelled using a series of ordinary differential rate equations, to simulate the obtained experimental results. The sensitivity of the model to the key equation parameters was also explored. The cellular glycolysis pathway kinetics were determined for three different cell-lines, under nonmodulated and modulated conditions. Over the timescale s

SUBMITTER: Patil N 

PROVIDER: S-EPMC10909989 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature