Intracellular Delivery of Functional Proteins with DNA-Protein Nanogels-Lipids Complex.

ABSTRACT: Using functional proteins for therapeutic purposes due to their high selectivity and/or catalytic properties can enable the control of various cellular processes; however, the transport of active proteins inside living cells remains a major challenge. In contrast, intracellular delivery of nucleic acids has become a routine method for a number of applications in gene therapy, genome editing, or immunization. Here we report a functionalizable platform constituting of DNA-protein nanogel carriers cross-linked through streptavidin-biotin or streptactin-biotin interactions and demonstrate its applicability for intracellular delivery of active proteins. We show that the nanogels can be loaded with proteins bearing either biotin, streptavidin, or strep-tag, and the resulting functionalized nanog

SUBMITTER: Mariconti M 

PROVIDER: S-EPMC10910493 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature