Induced pluripotent stem cell derived pericytes respond to mediators of proliferation and contractility.

ABSTRACT:

Background

Pericytes are multifunctional contractile cells that reside on capillaries. Pericytes are critical regulators of cerebral blood flow and blood-brain barrier function, and pericyte dysfunction may contribute to the pathophysiology of human neurological diseases including Alzheimers disease, multiple sclerosis, and stroke. Induced pluripotent stem cell (iPSC)-derived pericytes (iPericytes) are a promising tool for vascular research. However, it is unclear how iPericytes functionally compare to primary human brain vascular pericytes (HBVPs).

Methods

We differentiated iPSCs into iPericytes of either the mesoderm or neural crest lineage using established protocols. We compared iPericyte and HBVP morphologies, quantified gene expression by qPCR and bulk RNA sequencing,

SUBMITTER: King NE 

PROVIDER: S-EPMC10910734 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature