Crosstalk between keratinocytes and neutrophils shapes skin immunity against S. aureus infection.

ABSTRACT:

Introduction

Staphylococcus aureus (S. aureus) infection of the skin leads to a rapid initial innate immune response with keratinocytes in the epidermis as the initial sensors. Polymorphonuclear neutrophils (PMNs) are the first innate immune cells to infiltrate infection sites where they provide an effective first-line of defense. Previous work of our group showed that in inflamed skin a crosstalk between PMNs and keratinocytes results in enhanced S. aureus skin colonization.

Methods

In this work, we used an in vitro co-culture model to studied the crosstalk between primary human keratinocytes (PHKs) and PMNs in a sterile environment and upon S. aureus infection. We investigated the influence of PHKs on PMN activation by analyzing PMN lifespan, ex

SUBMITTER: Focken J 

PROVIDER: S-EPMC10911042 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature