CDKN2A promoter methylation enhances self-renewal of glioblastoma stem cells and confers resistance to carmustine.

ABSTRACT:

Background

Glioblastoma, a highly aggressive form of brain cancer, poses significant challenges due to its resistance to therapy and high recurrence rates. This study aimed to investigate the expression and functional implications of CDKN2A, a key tumor suppressor gene, in glioblastoma cells, building upon the existing background of knowledge in this field.

Method

Quantitative reverse transcription PCR (qRT-PCR) analysis was performed to evaluate CDKN2A expression in U87 glioblastoma cells compared to normal human astrocytes (NHA). CDKN2A expression levels were manipulated using small interfering RNA (siRNA) and CDKN2A overexpression vector. Cell viability assays and carmustine sensitivity tests were conducted to assess the impact of CDKN2A modulation on glioblastoma cell vi

SUBMITTER: Wang J 

PROVIDER: S-EPMC10912136 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature