In vivo interference of pea aphid endosymbiont Buchnera groEL gene by synthetic peptide nucleic acids.

ABSTRACT: The unculturable nature of intracellular obligate symbionts presents a significant challenge for elucidating gene functionality, necessitating the development of gene manipulation techniques. One of the best-studied obligate symbioses is that between aphids and the bacterial endosymbiont Buchnera aphidicola. Given the extensive genome reduction observed in Buchnera, the remaining genes are crucial for understanding the host-symbiont relationship, but a lack of tools for manipulating gene function in the endosymbiont has significantly impeded the exploration of the molecular mechanisms underlying this mutualism. In this study, we introduced a novel gene manipulation technique employing synthetic single-stranded peptide nucleic acids (PNAs). We targeted the critical Buchnera groEL using spec

SUBMITTER: Tan KXY 

PROVIDER: S-EPMC10912616 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature