A ubiquitous GC content signature underlies multimodal mRNA regulation by DDX3X.

ABSTRACT: The road from transcription to protein synthesis is paved with many obstacles, allowing for several modes of post-transcriptional regulation of gene expression. A fundamental player in mRNA biology is DDX3X, an RNA binding protein that canonically regulates mRNA translation. By monitoring dynamics of mRNA abundance and translation following DDX3X depletion, we observe stabilization of translationally suppressed mRNAs. We use interpretable statistical learning models to uncover GC content in the coding sequence as the major feature underlying RNA stabilization. This result corroborates GC content-related mRNA regulation detectable in other studies, including hundreds of ENCODE datasets and recent work focusing on mRNA dynamics in the cell cycle. We provide further evidence for mRNA stabiliz

SUBMITTER: Jowhar Z 

PROVIDER: S-EPMC10912769 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature