The population context is a driver of the heterogeneous response of epithelial cells to interferons.

ABSTRACT: Isogenic cells respond in a heterogeneous manner to interferon. Using a micropatterning approach combined with high-content imaging and spatial analyses, we characterized how the population context (position of a cell with respect to neighboring cells) of epithelial cells affects their response to interferons. We identified that cells at the edge of cellular colonies are more responsive than cells embedded within colonies. We determined that this spatial heterogeneity in interferon response resulted from the polarized basolateral interferon receptor distribution, making cells located in the center of cellular colonies less responsive to ectopic interferon stimulation. This was conserved across cell lines and primary cells originating from epithelial tissues. Importantly, cells embedded wit

SUBMITTER: Metz-Zumaran C 

PROVIDER: S-EPMC10912784 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature