Cycloheximide in the nanomolar range inhibits seed germination of Orobanche minor.

ABSTRACT: From the 992 samples of culture extracts of microorganisms isolated from soil in Japan, we found that the extract of Streptomyces sp. no. 226 inhibited Orobanche minor seed germination without significantly affecting the seed germination of Trifolium pratense and the growth of Aspergillus oryzae and Escherichia coli. Using ESI-MS, ¹H-NMR, and ¹³C-NMR, we identified the active compound as cycloheximide. Cycloheximide had half-maximum inhibitory concentrations of 2.6 ng/mL for the inhibition of seed germination of O. minor and 2.5 µg/mL for that of the conidial germination of A. oryzae. Since cycloheximide is known to inhibit translation by interacting with ribosomal protein L28 (RPL28) in yeast, we investigated wh

SUBMITTER: Nogami R 

PROVIDER: S-EPMC10912901 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature