Bacterial Lactonases ZenA with Noncanonical Structural Features Hydrolyze the Mycotoxin Zearalenone.

ABSTRACT: Zearalenone (ZEN) is a mycoestrogenic polyketide produced by Fusarium graminearum and other phytopathogenic members of the genus Fusarium. Contamination of cereals with ZEN is frequent, and hydrolytic detoxification with fungal lactonases has been explored. Here, we report the isolation of a bacterial strain, Rhodococcus erythropolis PFA D8-1, with ZEN hydrolyzing activity, cloning of the gene encoding α/β hydrolase ZenA encoded on the linear megaplasmid pSFRL1, and biochemical characterization of nine homologues. Furthermore, we report site-directed mutagenesis as well as structural analysis of the dimeric ZenA_Re of R. erythropolis and the more thermostable, tetrameric ZenA_Scfl of Streptomyces coelicoflavus with and without bound li

SUBMITTER: Fruhauf S 

PROVIDER: S-EPMC10913051 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature