Chromatograms and Mass Spectra of High-Mannose and Paucimannose N-Glycans for Rapid Isomeric Identifications.

ABSTRACT: N-Linked glycosylation is one of the most essential post-translational modifications of proteins. However, N-glycan structural determination remains challenging because of the small differences in structures between isomers. In this study, we constructed a database containing collision-induced dissociation MSⁿ mass spectra and chromatograms of high-performance liquid chromatography for the rapid identification of high-mannose and paucimannose N-glycan isomers. These N-glycans include isomers by breaking of arbitrary numbers of glycosidic bonds at arbitrary positions of canonical Man₉GlcNAc₂ N-glycans. In addition, some GlcMan_nGlcNAc₂ N-glycan isomers were included in the database. This d

SUBMITTER: Liew CY 

PROVIDER: S-EPMC10913092 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature