N⁶-methyladenosine levels in peripheral blood RNA: a potential diagnostic biomarker for colorectal cancer.

ABSTRACT:

Background

N⁶-methyladenosine (m⁶A) is dysregulated in various cancers, including colorectal cancer (CRC). Herein, we assess the diagnostic potential of peripheral blood (PB) m⁶A levels in CRC.

Methods

We collected PB from healthy controls (HCs) and patients with CRC, analyzed PB RNA m⁶A levels and the expression of m⁶A-related demethylase genes FTO and ALKBH5, cocultured CRC cells with PB mononuclear cells (PBMCs), and constructed an MC38 cancer model.

Results

PB RNA m⁶A levels were higher in the CRC than that in HCs. The area under the curve (AUC) of m⁶A levels (0.886) in the CRC was significantly larger compared with carbohydrate antigen 199 (CA199; 0.666) and carcinoembryonic antigen (CEA

SUBMITTER: Zhang C 

PROVIDER: S-EPMC10913687 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature