ChipFilter: Microfluidic-Based Comprehensive Sample Preparation Methodology for Microbial Consortia.

ABSTRACT: The metaproteomic approach is an attractive way to describe a microbiome at the functional level, allowing the identification and quantification of proteins across a broad dynamic range as well as the detection of post-translational modifications. However, it remains relatively underutilized, mainly due to technical challenges that should be addressed, including the complexity of extracting proteins from heterogeneous microbial communities. Here, we show that a ChipFilter microfluidic device coupled to a liquid chromatography tandem mass spectrometry (LC-MS/MS) setup can be successfully used for the identification of microbial proteins. Using cultures of Escherichia coli, Bacillus subtilis, and Saccharomyces cerevisiae, we have shown that it is possible to directly lys

SUBMITTER: Ravi Kumar RK 

PROVIDER: S-EPMC10913871 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature