Effects of self-assembled type II collagen fibrils on the morphology and growth of pre-chondrogenic ATDC5 cells.

ABSTRACT:

Objective

Although type II collagen could have marked potential for developing cartilage tissue engineering (CTE) scaffolds, its erratic supply and viscous nature have limited these studies, and there are no studies on the use of marine-derived type II collagen fibrils for CTE scaffold materials. In this study, we aimed to generate a fibril-based, thin-layered scaffold from marine-derived type II collagen and investigate its chondrogenic potential.

Methods

Time-lapse observations revealed the cell adhesion process. The Cell Counting Kit-8 (CCK-8) assay, light microscopy, and scanning electron microscopy were performed to detect proliferation and filopodium morphology. Alcian blue staining was used to show the deposition of extracellular secretions, and qRT-PCR was performed

SUBMITTER: Shi L 

PROVIDER: S-EPMC10914481 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature