FacZ is a GpsB-interacting protein that prevents aberrant division-site placement in Staphylococcus aureus.

ABSTRACT: Staphylococcus aureus is a Gram-positive pathogen responsible for antibiotic-resistant infections. To identify vulnerabilities in cell envelope biogenesis that may overcome resistance, we enriched for S. aureus transposon mutants with defects in cell surface integrity or cell division by sorting for cells that stain with propidium iodide or have increased light-scattering properties, respectively. Transposon sequencing of the sorted populations identified more than 20 previously uncharacterized factors impacting these processes. Cells inactivated for one of these proteins, factor preventing extra Z-rings (FacZ, SAOUHSC_01855), showed aberrant membrane invaginations and multiple FtsZ cytokinetic rings. These phenotypes were suppressed in mutants lacking the conserved cell-division protein G

SUBMITTER: Bartlett TM 

PROVIDER: S-EPMC10914604 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature