Heart rhythm in vitro: measuring stem cell-derived pacemaker cells on microelectrode arrays.

ABSTRACT:

Background

Cardiac arrhythmias have markedly increased in recent decades, highlighting the urgent need for appropriate test systems to evaluate the efficacy and safety of new pharmaceuticals and the potential side effects of established drugs.

Methods

The Microelectrode Array (MEA) system may be a suitable option, as it provides both real-time and non-invasive monitoring of cellular networks of spontaneously active cells. However, there is currently no commercially available cell source to apply this technology in the context of the cardiac conduction system (CCS). In response to this problem, our group has previously developed a protocol for the generation of pure functional cardiac pacemaker cells from mouse embryonic stem cells (ESCs). In addition, we compared the hanging

SUBMITTER: Kussauer S 

PROVIDER: S-EPMC10915086 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature