DEC1 is involved in TGF-β1-induced epithelial-mesenchymal transition of gastric cancer.

ABSTRACT: DEC1 is a helix-loop-helix (bHLH) transcription factor, whose deregulation has been observed in several tumors. However, the effects of the dysregulation of this gene on epithelial-mesenchymal transition (EMT) are controversial, with its roles in gastric cancer (GC) remaining unclear. In the present study, we focused on the impact of DEC1 on EMT and cell mobility in gastric cancer. We found that DEC1 expression positively correlated with TGF-β1 and EMT markers in tumor issues, and that DEC1 facilitated TGF-β1-induced EMT in gastric cancer. In addition, gastric cancer cell migration potential was reduced after DEC1 knockdown. Using murine metastasis models, we confirmed that DEC1 promoted GC metastasis and further explored the correlation of DEC1 with TGF-β1 and E-cadherin in vivo. Chromatin immunoprecipitation (ChIP) assays revealed that DEC1 could directly interact with the promoter region of TGF-β1. These results suggest that DEC1 functions as a tumor enhancer that partially participates in TGF-β1-mediated EMT processes in GC, thus contributing to tumor metastasis.

SUBMITTER: Li P 

PROVIDER: S-EPMC10915339 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature