Correlative single-cell hard X-ray computed tomography and X-ray fluorescence imaging.

ABSTRACT: X-ray computed tomography (XCT) and X-ray fluorescence (XRF) imaging are two non-invasive imaging techniques to study cellular structures and chemical element distributions, respectively. However, correlative X-ray computed tomography and fluorescence imaging for the same cell have yet to be routinely realized due to challenges in sample preparation and X-ray radiation damage. Here we report an integrated experimental and computational workflow for achieving correlative multi-modality X-ray imaging of a single cell. The method consists of the preparation of radiation-resistant single-cell samples using live-cell imaging-assisted chemical fixation and freeze-drying procedures, targeting and labeling cells for correlative XCT and XRF measurement, and computational reconstruction of the corre

SUBMITTER: Lin Z 

PROVIDER: S-EPMC10917812 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature