The serine/threonine protein kinase MpSTE1 directly governs hyphal branching in Monascus spp.

ABSTRACT: Monascus spp. are commercially important fungi due to their ability to produce beneficial secondary metabolites such as the cholesterol-lowering agent lovastatin and natural food colorants azaphilone pigments. Although hyphal branching intensively influenced the production of these secondary metabolites, the pivotal regulators of hyphal development in Monascus spp. remain unclear. To identify these important regulators, we developed an artificial intelligence (AI)-assisted image analysis tool for quantification of hyphae-branching and constructed a random T-DNA insertion library. High-throughput screening revealed that a STE kinase, MpSTE1, was considered as a key regulator of hyphal branching based on the hyphal phenotype. To further validate the role of MpSTE1, we generated an mpSTE1 gen

SUBMITTER: Duan Y 

PROVIDER: S-EPMC10917826 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature