Analyzing Thalamocortical Tract-Tracing Experiments in a Common Reference Space.

ABSTRACT: Current mesoscale connectivity atlases provide limited information about the organization of thalamocortical projections in the mouse brain. Labeling the projections of spatially restricted neuron populations in thalamus can provide a functionally relevant level of connectomic analysis, but these need to be integrated within the same common reference space. Here, we present a pipeline for the segmentation, registration, integration and analysis of multiple tract-tracing experiments. The key difference with other workflows is that the data is transformed to fit the reference template. As a test-case, we investigated the axonal projections and intranuclear arrangement of seven neuronal populations of the ventral posteromedial nucleus of the thalamus (VPM), which we labeled with an anterograd

SUBMITTER: Timonidis N 

PROVIDER: S-EPMC10917831 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature