Detection of donor-derived cell-free DNA in the setting of multiple kidney transplantations.

ABSTRACT:

Background

The routine use of donor-derived cell-free DNA (dd-cfDNA) assays to monitor graft damage in patients after kidney transplantation is being implemented in many transplant centers worldwide. The interpretation of the results can be complicated in the setting of multiple sequential kidney transplantations where accurate donor assignment of the detected dd-cfDNA can be methodologically challenging.

Methods

We investigated the ability of a new next-generation sequencing (NGS)-based dd-cfDNA assay to accurately identify the source of the detected dd-cfDNA in artificially generated samples as well as clinical samples from 31 patients who had undergone two sequential kidney transplantations.

Results

The assay showed a high accuracy in quantifying and correctly assi

SUBMITTER: Pettersson L 

PROVIDER: S-EPMC10917974 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature